Many remote-entanglement protocols rely on the generation and interference of photons produced by nodes within a quantum network. Quantum networks based on heterogeneous nodes provide a versatile platform by utilizing the complementary strengths of the differing systems. Implementation of such networks is challenging, due to the disparate spectral and temporal characteristics of the photons generated by the different quantum systems. Here, we report on the observation of quantum interference between photons generated from a single ion and an atomic ensemble. The photons are produced on demand by each source located in separate buildings, in a manner suitable for quantum networking. Given these results, we analyze the feasibility of hybrid ion-ensemble remote entanglement generation.